n the agricultural sector and in particular the cereal chain of high quality durum wheat, it is necessary to define a methodology based on scientific evidence, to resolve some issues regarding the sustainability of the agricultural sector of the crop. In particular, it is necessary to provide for the establishment of appropriate experimental protocols in field and laboratory applications of management techniques and investigation of various physiological aspects of crop and soil quality, the impacts of nitrogen nutrition, in different scenarios integrated crop management (conventional and conservative), bioagronomical and productive aspects, but also on many aspects of some quality parameters of grain, food safety and the environment. Relationship between nutrition and nitrogen dynamics of accumulation of some important molecules for grain quality, the identification of those traits most correlated with the nutritional status of the crop, and the influence of agronomic practices against accumulation of harmful substances for human consumption such as mycotoxins and heavy metals.
The research activities will be complemented by laboratory testing, the kernels still maturing, until fully ripe, will study the dynamics of protein accumulation, and glutinico important antioxidant molecules and the identification and quantification of amino acids. Will be applied to protein extraction procedures and the entire proteome, followed by laboratory analysis with the Proteome Lab PF2D (Beckman Coulter) for a chromatographic separation of proteins in two dimensions. The proteins are fractionated and characterized by MALDI-TOF MS (Matrix Assisted Laser desorption Ionization Mass Spectrometry). The grain will mature routine qualitative analysis carried out (weight of thousand seeds, the test weight, fiber content, carotenoid pigments and glass component) as well as the digestibility of starch.