Conservation and more particularly to the cryopreservation of the semen is a reproductive technology subject of continuous study. Numerous procedures for storage and cryopreservation of sperm were studied over the years with the aim of improving the viability and fertilizing capacity of spermatozoa after cryopreservation. However, the freezing of semen in the poultry industry is still a very common reproductive technique because of the lack of standardized procedures for freezing able to ensure an adequate level of fertility of sperm after thawing. Although the first experiments were conducted in 1951 in the chicken (Polge, 1951), in the years following protocols have been developed on the cryopreservation of semen without achieving satisfactory results as in other species that can ensure a good survival of male gametes (Bellagamba et al, 1993; Donoghue and Wishart, 2000).

Solution provided by this research is the study of the characteristics of turkey semen by phase contrast microscopy to evaluate sperm concentration, motility, and morphology using particular colors ensuring the preparation of a classification of morphological changes frequently

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